Allele-Specific PCR for Determination of IL28B Genotype
نویسندگان
چکیده
منابع مشابه
Allele-specific PCR for determination of IL28B genotype.
The IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.
متن کاملDetermination of the TLR4 genotype using allele-specific PCR.
Despite significant advances in understanding the molecular basis of sepsis and its associated immunological response, sepsis remains a problem worldwide and is associated with a high mortality. Annually, septic shock, the most severe form of sepsis, causes the death of more than 100 000 people in the USA (3,4). Gram-negative bacteria are the most common pathogens associated with bacterial infe...
متن کاملCLN3 by allele specific PCR
The recent isolation of the CLN3 gene involved in Batten disease (juvenile neuronal ceroid lipofuscinosis) creates possibilities for direct detection of mutations which can confirm or indicate the clinical diagnosis of Batten disease. We have designed a rapid and reliable allele specific PCR test for the detection of the major deletion, which can be used in carrier diagnosis, presymptomatic dia...
متن کاملAllele and Genotype Frequency of IL28B (rs12979860) in South Iranian population
Hepatitis C virus (HCV) has chronically infected million peoples worldwide. Spontaneous viral clearance occurs in 25% of affected patients. The standard regimen to treat HCV infection is the combination of pegylated interferon and ribavirin.1 The most important indicator of successful treatment is sustained virologic response (SVR), which is defined as undetectable HCV RNA 24 weeks after treatm...
متن کاملS-genotype identification based on allele-specific PCR in Japanese pear
Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2012
ISSN: 0095-1137
DOI: 10.1128/jcm.02084-12